A new paper about qPCR for cyanobacterial geosmin/MIB detection in drinking water was published by Devi et al. in Water Research.
Highlights (from paper):
An online database (CyanoGM Explorer) for global geosmin/2-MIB events is created.
Quantitative PCR (qPCR) provides rapid, reliable and early on-site detection tool.
Universal primer/probe for identifying 58 available geo sequences is lacking.
2-3 out of 11 developed primer/probe sets can detect all 28 available mic sequences.
Primer design/optimization, field validation and qPCR protocols are key challenges.
Reference:
Apramita Devi, Yi-Ting Chiu, Hsin-Ta Hsueh, Tsair-Fuh Lin, Quantitative PCR based detection system for cyanobacterial geosmin/2-methylisoborneol (2-MIB) events in drinking water sources: Current status and challenges. Water Research, Volume 188,
2021, https://doi.org/10.1016/j.watres.2020.116478.
WaterTOP COST Action (CA18225) 